| 1. | Then pseudomonas sp genomic library was constructed
阳性克隆的筛选工作仍在进行中。 |
| 2. | The me2155 genomic library was constructed using phz132 as the cosmid vector , and 10 positive clones were fished out using 0 . 9kb pcr product as probe
以phz132为载体，构建了mc ~ 2155的基因组文库。以0 . 9kb的扩增产物为探针从文库中钓出10个阳性克隆子。 |
| 3. | Screening of a potato genomic library with a full - length sb401 cdna resulted in the isolation of six positive clones . one of clones , designated 901 , was further characterized
对花粉发育过程中特异表达基因的研究，科学家们希望从分子水平上阐明小孢子发育的本质。 |
| 4. | Through in situ hybridization and colony pcr , a positive recombinant plasmid was isolated from the genomic library and sequenced . the inserted dna fragment was about 4 . 3 kb
将pcr扩增片段用地高辛标记探针，利用原位杂交和菌落pcr从基因文库中获得含有阳性信号的重组质粒。 |
| 5. | In the past , we cloned the cdna of zmcdc5 based on the studies on difference of gene expression in shoot and radicle of maize seedling in our lab and then got the whole genome sequence by maize genomic library screening and inverse pcr
本实验室根据玉米胚芽和胚根差异表达的研究，克隆得到zmcdc5基因的cdna ，并通过筛库和反向pcr获得了zmcdc5基因组全长序列。 |
| 6. | Its genomic dna was partially digested by sauial . dna fragments from 4 to 16 kb were collected after electrophoresis and ligated with bamhi - digested puc18 to construct genomic library . the total number of recombinant plasmids is about 9000
进一步在opua基因的上下游序列分别设计引物，从h . trueperi基因组dna中扩增出所预期大小的片段，测序验证已获得opua基因的全序列。 |
| 7. | When cotransforming pgbd - nifa and ad fusion genomic library into saccharomyces cerevisiae pj69 - 4a , 109 candidates interacting with nifa had been selected by testing for the expression of the his3 , ade2 and lacz reporter genes
诱饵质粒和文库质粒共转化酿酒酵母( saccharomycescerevisiae ) pj69 - 4a ，通过检测报告基因his3 、 ade2及lacz的表达进行筛选，初筛得到109个阳性酵母菌落。 |
| 8. | In this paper several methods of developing microsatellite primers in plants were summarized : to search the database and find the primers which were delivered ; to transfer them among the different species ; to establish genomic library ; to develop ssr primer based on moleculars markers
摘要综述了开发植物微卫星引物的几种方法：搜寻核酸数据库和寻找已存在的微卫星引物；不同物种间共用；建立基因组文库；利用分子标记技术发展ssr引物。 |
| 9. | Pfge analysis of these 21 blocked strains revealed a common chromosomal deletion in the 300kb asel fragment which might be responsible for antibiotic 5102 - iii biosynthesis . so the 300kb fragment was recovered and used as probe to hybridize with 10 - 22 genomic library . cosmids in this region were aligned and suitable fragments in this region were selected and used further for the construction of gene replacement plasmids
以此片段为探针钓出了位于该区域的文库克隆，并利用指纹印迹和杂交技术将这些文库克隆排列起来，进而以位于该区域的不同位置的片段做臂构建了用于转化和接合转移用的基因置换质粒，并试图通过基因置换将该区域置换下来，但尚未得到最终结果。 |
| 10. | The library consisted of 1 . 3 x 106 clones with an average insert size of about 18kb . the capacity of this library was about 20 times the equivalent of the genome of atriplex centralasiatica iljin . screening the genomic library with a 400bp probe located at the 5 ' end of the badh gene obtained by rt - pcr , we got four positive clones
3x10 ‘个重组噬菌体，插入片段大小约为18kb ，含插入片段的频率为100队以中亚滨蓉甜菜碱醛脱氢酶门adh ）基因近5 ’端的约400hp片段为探针，筛选中亚滨蓉基因组文库，得到了4个阳性克隆。 |
